FGF-5, human recombinant protein
Fibroblast Growth Factor-5, HBGF-5, Smag-82
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P12034 |
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Calculated MW | 27.6 kDa |
Gene ID | 2250 |
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Gene Symbol | FGF5 |
Other Names | Fibroblast Growth Factor-5, HBGF-5, Smag-82 |
Gene Source | Human |
Source | E.coli |
Assay&Purity | SDS-PAGE; ≥95% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Sequence | MAWAHGEKRL APKGQPGPAA TDRNPIGSSS RQSSSSAMSS SSASSSPAAS LGSQGSGLEQ SSFQWSPSGR RTGSLYCRVG IGFHLQIYPD GKVNGSHEAN MLSVLEIFAV SQGIVGIRGV FSNKFLAMSK KGKLHASAKF TDDCKFRERF QENSYNTYAS AIHRTEKTGR EWYVALNKRG KAKRGCSPRV KPQHISTHFL PRFKQSEQPE LSFTVTVPEK KNPPSPIKSK IPLSAPRKNT NSVKYRLKFR FG |
Target/Specificity | FGF-5 |
Application Notes | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
Format | Lyophilized powder |
Storage | -20°C; Sterile filtered through a 0.2 micron filter. Lyophilized from 5 mM Tris, pH 7.5, and 25 mM NaCl. |
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Provided below are standard protocols that you may find useful for product applications.
Background
FGF-5 is a secreted heparin binding growth factor that belongs to the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-5 binds to FGFR 1c and 2c, and plays a regulatory role in the hair growth cycle. Recombinant human FGF-5 is a 27.6 kDa protein consisting of 252 amino acid residues.
References
Haub O.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:8022-8026(1990).
Zhan X.,et al.Mol. Cell. Biol. 8:3487-3495(1988).
Ozawa K.,et al.Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases.
de Vries C.J.M.,et al.J. Biol. Chem. 275:23939-23947(2000).
Hanada K.-I.,et al.Cancer Res. 61:5511-5516(2001).
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