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GASP-1, human recombinant protein

GDF-associated serum protein-1, GASP, KIAA0443, WFIKKNRP

     
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Product info
Primary Accession Q5JY77
Calculated MW 55-66 kDa
Additional Info
Gene ID 9737
Gene Symbol GASP
Other Names GDF-associated serum protein-1, GASP, KIAA0443, WFIKKNRP
Gene Source Human
Source CHO cells
Assay&Purity SDS-PAGE; ≥95%
Assay2&Purity2 HPLC;
Recombinant Yes
Sequence LPPIRYSHAG ICPNDMNPNL WVDAQSTCRR ECETDQECET YEKCCPNVCG TKSCVAARYM DVKGKKGPVG MPKEATCDHF MCLQQGSECD IWDGQPVCKC KDRCEKEPSF TCASDGLTYY NRCYMDAEAC SKGITLAVVT CRYHFTWPNT SPPPPETTMH PTTASPETPE LDMAAPALLN NPVHQSVTMG ETVSFLCDVV GRPRPEITWE KQLEDRENVV MRPNHVRGNV VVTNIAQLVI YNAQLQDAGI YTCTARNVAG VLRADFPLSV VRGHQAAATS ESSPNGTAFP AAECLKPPDS EDCGEEQTRW HFDAQANNCL TFTFGHCHRN LNHFETYEAC MLACMSGPLA ACSLPALQGP CKAYAPRWAY NSQTGQCQSF VYGGCEGNGN NFESREACEE SCPFPRGNQR CRACKPRQKL VTSFCRSDFV ILGRVSELTE EPDSGRALVT VDEVLKDEKM GLKFLGQEPL EVTLLHVDWA CPCPNVTVSE MPLIIMGEVD GGMAMLRPDS FVGASSARRV RKLREVMHKK TCDVLKEFLG LH
Target/Specificity GASP-1
Application Notes Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Format Lyophilized powder
Storage -20°C; Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM sodium phosphate, pH 7.8.
Citations (0)

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Background

Growth and differentiation factor-associated serum protein-1 (GASP-1) is a secreted inhibitory TGF-β binding protein that contains multiple protease inhibitor structural domains. It is expressed primarily in the ovary, testis, and brain, and can act as a potent soluble inhibitor of myostatin and GDF-11, but not Activin-A. The GASP-1 gene encodes a 571 amino acid protein that contains a 29 amino acid secretion signal sequence, and multiple identifiable structural features, including a WAP domain, a follistatin/Kazal domain, an immunoglobulin domain, two tandem Kunitz domains, and a netrin domain. Recombinant human GASP-1 is a 542 amino acid protein that migrates at an apparent molecular weight of approximately 55-66 kDa by SDS-PAGE analysis under non-reducing conditions.

References

Ishikawa K.,et al.DNA Res. 4:307-313(1997).
Ross M.T.,et al.Nature 434:325-337(2005).
Patzak D.,et al.Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases.
Whistler J.L.,et al.Science 297:615-620(2002).
Heydorn A.,et al.J. Biol. Chem. 279:54291-54303(2004).

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$ 165.00
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Cat# PBV10782r
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