|Calculated MW||13.3 kDa|
|Other Names||MK, NEGF-2|
|Sequence||VAKKKEKVKK GSECSEWTWG PCTPSSKDCG MGFREGTCGA QTQRVHCKVP CNWKKEFGAD CKYKFESWGA CDGSTGTKAR QGTLKKARYN AQCQETIRVT KPCTSKTKSK TKAKKGKGKD|
|Application Notes||Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.|
|Storage||-20°C; Sterile filtered through a 0.2 micron filter. Lyophilized from 0.4 x PBS, pH 7.4.|
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Provided below are standard protocols that you may find useful for product applications.
Midkine and its functionally-related protein Pleiotrophin are heparin-binding neurotrophic factors that signal through the same receptor, known as anaplastic lymphoma kinase (ALK). MK plays an important regulatory role in epithelial-mesenchymal interactions during fetal development and in postnatal lung development. MK chemoattracts embryonic neurons, neutrophils and macrophages, and by signaling through the ALK receptor it exerts angiogenic, growth and survival activities during tumorigenesis. Recombinant murine Midkine is a 13.3 kDa protein containing 120 amino acid residues including five intra-molecular disulfide bonds.
Matsubara S.,et al.J. Biol. Chem. 265:9441-9443(1990).
Tomomura M.,et al.J. Biol. Chem. 265:10765-10770(1990).
Kadomatsu K.,et al.Biochem. Biophys. Res. Commun. 151:1312-1318(1988).
Kaneda N.,et al.J. Biochem. 119:1150-1156(1996).
Horiba M.,et al.J. Clin. Invest. 105:489-495(2000).
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