|Calculated MW||9.3 kDa|
|Other Names||Monocyte Chemotactic Protein-5, CCL12|
|Sequence||GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG|
|Application Notes||Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.|
|Storage||-20°C; Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.|
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Provided below are standard protocols that you may find useful for product applications.
The MCP proteins belong to the CC chemokine family, and signal through CCR2 and, with the exception of MCP-1, other CCR receptors. The MCP proteins chemoattract and activate monocytes, activated T cells, basophils, NK cells, and immature dendritic cells. The MCP family cross- reacts across species. Recombinant murine MCP-5 is a 9.3 kDa protein containing 82 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines.
Jia G.-Q.,et al.J. Exp. Med. 184:1939-1951(1996).
Sarafi M.N.,et al.J. Exp. Med. 185:99-109(1997).
Teuscher C.,et al.J. Immunol. 163:2262-2266(1999).
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