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MEC/CCL28, murine recombinant protein
Mucosae-associated Epithelial Chemokine, CCL28, CCK-1
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q9JIL2 |
|---|---|
| Calculated MW | 12.6 kDa |
| Gene ID | 56838 |
|---|---|
| Gene Symbol | CCL28 |
| Other Names | Mucosae-associated Epithelial Chemokine, CCL28, CCK-1 |
| Gene Source | Murine |
| Source | E. Coli |
| Assay&Purity | SDS-PAGE; ≥98% |
| Assay2&Purity2 | HPLC; |
| Recombinant | Yes |
| Sequence | SEAILPMASS CCTEVSHHVS GRLLERVSSC SIQRADGDCD LAAVILHVKR RRICISPHNR TLKQWMRASE VKKNGRENVC SGKKQPSRKD RKGHTTRKHR TRGTHRHEAS R |
| Target/Specificity | CCL28 |
| Application Notes | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
| Format | Lyophilized powder |
| Storage | -20°C; Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Citrate, pH 4.0. |
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Provided below are standard protocols that you may find useful for product applications.
Background
MEC is a secreted CC chemokine expressed primarily by epithelial cells of the bronchioles, salivary gland, mammary gland and colon. MEC signals through the CCR10 receptor and chemoattracts resting CD4, CD8 T cells and eosinophils. MEC contains six cysteines including the four highly conserved cysteine residues present in CC chemokines. Recombinant murine MEC is a 12.6 kDa protein containing 111 amino acid residues.
References
Wang W.,et al.J. Biol. Chem. 275:22313-22323(2000).
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