|Calculated MW||11.3 kDa|
|Other Names||Macrophage Inflammatory Protein-3, CCL23, MPIF-1, Ck-β8|
|Sequence||RVTKDAETEF MMSKLPLENP VLLDRFHATS ADCCISYTPR SIPCSLLESY FETNSECSKP GVIFLTKKGR RFCANPSDKQ VQVCMRMLKL DTRIKTRKN|
|Application Notes||Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.|
|Storage||-20°C; Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.|
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Provided below are standard protocols that you may find useful for product applications.
MIP-3 is a CC chemokine that signals through the CCR1 receptor. MIP-3 chemoattracts monocytes, resting T-lymphocytes and neutrophils, but does not chemoattract activated lymphocytes. Additionally, MIP-3 has been shown to inhibit colony formation of bone marrow myeloid immature progenitors. Recombinant human MIP-3 is an 11.3 kDa protein containing 99 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.
Li H.,et al.Patent number US5504003, 02-APR-1996.
Patel V.P.,et al.J. Exp. Med. 185:1163-1172(1997).
Youn B.-S.,et al.Blood 91:3118-3126(1998).
Nomiyama H.,et al.J. Interferon Cytokine Res. 19:227-234(1999).
Zody M.C.,et al.Nature 440:1045-1049(2006).
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