Nesfatin-1, human recombinant protein
Nesfatin 1
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P80303 |
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Calculated MW | 9.7 kDa |
Gene ID | 4925 |
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Gene Symbol | NEFA |
Other Names | Nesfatin 1 |
Gene Source | Human |
Source | E. Coli |
Assay&Purity | SDS-PAGE; ≥98% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Sequence | VPIDIDKTKV QNIHPVESAK IEPPDTGLYY DEYLKQVIDV LETDKHFREK LQKADIEEIK SGRLSKELDL VSHHVRTKLD EL |
Target/Specificity | Nesfatin-1 |
Application Notes | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
Format | Lyophilized powder |
Storage | -20°C; Sterile filtered through a 0.2 micron filter. Lyophilized from 0.1% TFA |
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Provided below are standard protocols that you may find useful for product applications.
Background
Nesfatin-1 is a metabolic polypeptide encoded in the N-terminal region of the precursor protein, Nucleobindin2 (NUCB2). Originally identified as a hypothalamic neuropeptide, Nesfatin-1 is also expressed in other areas of the brain, and in pancreatic islets β-cells, gastric endocrine cells and adipocytes. Nesfatin-1 suppresses food intake and can regulate energy metabolism in a Leptin independent manner. Recombinant human Nesfatin-1 is a 9.7 kDa protein containing 82 amino acid residues.
References
Barnikol-Watanabe S.,et al.Biol. Chem. Hoppe-Seyler 375:497-512(1994).
Deangelis M.M.,et al.Biochim. Biophys. Acta 1407:84-91(1998).
Line A.,et al.Br. J. Cancer 86:1824-1830(2002).
Yamada M.,et al.Endocrinology 151:2494-2503(2010).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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