|Calculated MW||The protein is fused with 6×His tag at the C-terminus, has a calculated MW of 15.7 kDa. The predicted N-terminus is Met 1. DTT-reduced Protein migrates as 16 kDa.|
|Other Names||FABP3, H-FABP, FABP11, MDGI, M-FABP, hFABP, O-FABP|
|Source||HEK 293 cells|
|Application Notes||Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.|
|Storage||-20°C; Lyophilized from 0.22 µm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.|
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Provided below are standard protocols that you may find useful for product applications.
Fatty acid-binding protein 3 (FABP3) also known as Heart-type fatty acid binding protein (H-FABP), Mammary-derived growth inhibitor (MDGI), Muscle fatty acid-binding protein (M-FABP), FABP11, which belongs to the calycin superfamily and fatty-acid binding protein (FABP) family. FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. H-FABP / FABP3 is involved in active fatty acid metabolism where it transports fatty acids from the cell membrane to mitochondria for oxidation. FABP3 may also contribute to AS160 phosphorylation by maintaining insulin-dependent Akt activation in the cells under a lipotoxic condition.
Peeter R.A.,et al.Biochem. J. 276:203-207(1991).
Hu Y.F.,et al.Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases.
Wu X.,et al.Submitted (NOV-1994) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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