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Human CellExp HMGB1 /HMG1, human recombinant protein
HMGB1, HMG1, HMG3, SBP-1
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P09429 |
|---|---|
| Calculated MW | The protein is fused with 6×His tag at the C-terminus, has a calculated MW of 25.7 kDa. The predicted N-terminus is Met1. DTT-reduced Protein migrates as 28 kDa and 32 kDa due to different glycosylation. |
| Gene ID | 3146 |
|---|---|
| Gene Symbol | HMGB1 |
| Other Names | HMGB1, HMG1, HMG3, SBP-1 |
| Gene Source | Human |
| Source | HEK 293 cells |
| Assay&Purity | SDS-PAGE; ≥95% |
| Assay2&Purity2 | HPLC; |
| Recombinant | Yes |
| Target/Specificity | HMGB1 |
| Application Notes | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| Format | Lyophilized powder |
| Storage | -20°C; Lyophilized from 0.22 µm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization. |
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Provided below are standard protocols that you may find useful for product applications.
Background
High-mobility group protein B1 (HMGB1), also known as high-mobility group protein 1 (HMG-1) and amphoterin, is a member of the HMGB family consisting of three members, HMGB1, HMGB2 and HMGB3. HMGB1 is a non-histone architectural chromosomal protein ubiquitously present in all vertebrate nuclei and binds double-stranded DNA without sequence specificity. The mechanism of inflammation and damage is binding to TLR4, which mediates HMGB1-dependent activation of macrophage cytokine release. This positions HMGB1 at the intersection of sterile and infectious inflammatory responses. HMGB1 has been studied as a DNA vaccine adjuvant and a target for cancer therapy.
References
Wen L.,et al.Nucleic Acids Res. 17:1197-1214(1989).
Ferrari S.,et al.Genomics 35:367-371(1996).
Xiang Y.-Y.,et al.Int. J. Cancer 74:1-6(1997).
Kornblit B.,et al.Tissue Antigens 70:151-156(2007).
He F.T.,et al.Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases.
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