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CCL3L1, human recombinant protein
C-C motif chemokine 3-like 1, CCL3L3, D17S1718, G0S19-2, LD78, LD78BETA, MIP1AP, SCYA3L; SCYA3L1
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P16619 |
|---|---|
| Concentration | 0.25 |
| Calculated MW | 10 kDa (90 aa, 26-93 aa + His tag), confirmed by MALDI-TOF. |
| Gene ID | 6349 |
|---|---|
| Gene Symbol | CCL3L1 |
| Other Names | C-C motif chemokine 3-like 1, CCL3L3, D17S1718, G0S19-2, LD78, LD78BETA, MIP1AP, SCYA3L; SCYA3L1 |
| Gene Source | Human |
| Source | E. coli |
| Assay&Purity | SDS-PAGE; ≥90% |
| Assay2&Purity2 | N/A; |
| Recombinant | Yes |
| Sequence | MGSSHHHHHH SSGLVPRGSH MSLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPSV IFLTKRGRQV CADPSEEWVQ KYVSDLELSA |
| Target/Specificity | CCL3L1 |
| Format | Liquid |
| Storage | -80°C; 0.25 mg/ml in Phosphate Buffered Saline pH 7.4 containing 10% glycerol |
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Provided below are standard protocols that you may find useful for product applications.
Background
CCL3L1 is a small cytokine belonging to the CC chemokine. This protein is involved in immunoregulatory and inflammatory processes. It binds to several chemokine receptors including chemokine binding protein 2 (CCBP2 or D6) and chemokine (C-C motif) receptor 5 (CCR5). Recombinant human CCL3L1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
References
Irving S.G.,et al.Nucleic Acids Res. 18:3261-3270(1990).
Blum S.,et al.DNA Cell Biol. 9:589-602(1990).
Nakao M.,et al.Mol. Cell. Biol. 10:3646-3658(1990).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Zhang Z.,et al.Protein Sci. 13:2819-2824(2004).
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