|Calculated MW||24.8 kDa (225 aa, 31-232 aa + His tag), confirmed by MALDI-TOF.|
|Other Names||CD200 molecule, MOX1, MOX2, MRC, OX-2|
|Sequence||MGSSHHHHHH SSGLVPRGSH MGSQVQVVTQ DEREQLYTPA SLKCSLQNAQ EALIVTWQKK KAVSPENMVT FSENHGVVIQ PAYKDKINIT QLGLQNSTIT FWNITLEDEG CYMCLFNTFG FGKISGTACL TVYVQPIVSL HYKFSEDHLN ITCSATARPA PMVFWKVPRS GIENSTVTLS HPNGTTSVTS ILHIKDPKNQ VGKEVICQVL HLGTVTDFKQ TVNKG|
|Storage||-80°C; 1 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea.|
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Provided below are standard protocols that you may find useful for product applications.
CD200 is a type-1 membrane glycoprotein, which contains two immunoglobulin domains, and thus belongs to the immunoglobulin superfamily. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. Multiple alternatively spliced transcript variants that encode different isoforms have been found for this gene. Recombinant human CD200 protein, fused to His-tag at N-terminus, was expressed in E.coli.
Hou Z.F.,et al.Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
Mao Y.M.,et al.Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Otsuki T.,et al.DNA Res. 12:117-126(2005).
Muzny D.M.,et al.Nature 440:1194-1198(2006).
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