|Calculated MW||15 kDa (133 aa, 1-113 aa + His tag), confirmed by MALDI-TOF.|
|Other Names||Interferon regulatory factor-2|
|Sequence||MGSSHHHHHH SSGLVPRGSH MPVERMRMRP WLEEQINSNT IPGLKWLNKE KKIFQIPWMH AARHGWDVEK DAPLFRNWAI HTGKHQPGVD KPDPKTWKAN FRCAMNSLPD IEEVKDKSIK KGNNAFRVYR MLP|
|Storage||-80°C; 1 mg/ml in 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT and 10% glycerol.|
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Provided below are standard protocols that you may find useful for product applications.
Interferon regulatory factor (IRF) 2 is a member of the interferon regulatory transcription factor family. IRF-2 is generally regarded as an oncoprotein. Structure of IRF1 and IRF2 are similar. However, its functions are different. IRF2 competitively inhibits the IRF1-mediated transcriptional activation of interferons alpha and beta, and presumably other genes that employ IRF1 for transcription activation. Recombinant human IRF2 was expressed in E.coli and purified by using conventional chromatography techniques.
Itoh S.,et al.Nucleic Acids Res. 17:8372-8372(1989).
Cha Y.,et al.J. Biol. Chem. 269:5279-5287(1994).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
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