|Calculated MW||20.4 kDa (178 aa, 36-192 aa + His Tag), confirmed by MALDI-TOF.|
|Other Names||IL-18, IFN-gamma-inducing factor (IGIF), Interleukin-1 gamma|
|Sequence||MGSSHHHHHH SSGLVPRGSH MNFGRLHCTT AVIRNINDQV LFVDKRQPVF EDMTDIDQSA SEPQTRLIIY MYKDSEVRGL AVTLSVKDSK MSTLSCKNKI ISFEEMDPPE NIDDIQSDLI FFQKRVPGHN KMEFESSLYE GHFLACQKED DAFKLILKKK DENGDKSVMF TLTNLHQS|
|Storage||-80°C; 1 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol.|
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Provided below are standard protocols that you may find useful for product applications.
Interleukin 18 (IL-18), previously known as interferon-gamma (IFN-gamma)-inducing factor, is an activator of NK cells and a co-inducer of Th1 cytokines. IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IL-18 was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Okamura H.,et al.Nature 378:88-91(1995).
Rothe H.,et al.J. Clin. Invest. 99:469-474(1997).
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