|Calculated MW||36.2 kDa (321 aa, 17-314 aa + His Tag), confirmed by MALDI-TOF. (MW on SDS-PAGE shifts up)|
|Other Names||OPN, SPP-1, BNSP, BSPI, ETA-1|
|Sequence||MGSSHHHHHH SSGLVPRGSH RSMIPVKQAD SGSSEEKQLY NKYPDAVATW LNPDPSQKQN LLAPQNAVSS EETNDFKQET LPSKSNESHD HMDDMDDEDD DDHVDSQDSI DSNDSDDVDD TDDSHQSDES HHSDESDELV TDFPTDLPAT EVFTPVVPTV DTYDGRGDSV VYGLRSKSKK FRRPDIQYPD ATDEDITSHM ESEELNGAYK AIPVAQDLNA PSDWDSRGKD SYETSQLDDQ SAETHSHKQS RLYKRKANDE SNEHSDVIDS QELSKVSREF HSHEFHSHED MLVVDPKSKE EDKHLKFRIS HELDSASSEV N|
|Storage||-80°C; 1 mg/ml in 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol, and 2 mM EDTA.|
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Provided below are standard protocols that you may find useful for product applications.
Osteopontin (OPN), also known as Secreted phosphoprotein 1 (SPP1), is a human gene product which is conserved in other species. This protein is glycoprotein that was first identified in osteoblasts and plays an important role in bone remodeling, immune functions in fibroblasts, macrophages, and lymphocytes during inflammation and wound healing. Recombinant human Osteopontin protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
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