|Calculated MW||25.1 kDa (225 aa, 1-217 aa + His Tag), confirmed by MALDI-TOF. (MW on SDS-PAGE shifts up)|
|Other Names||Osteoclast stimulating factor 1, SH3P2, OSF|
|Sequence||MSKPPPKPVK PGEGGQVKVF RALYTFEPRT PDELYFEEGD IIYITDMSDT NWWKGTSKGR TGLIPSNYVA EQAESIDNPL HEAAKRGNLS WLRECLDNRV GVNGLDKAGS TALYWACHGG HKDIVEMLFT QPNIELNQQN KLGDTALHAA AWKGYADIVQ LFLAKGARTD LRNIEKKLAF DMATNAACAS LLKKKQGTDA VRTLSNAEDY LDDEDSDLEH HHHHH|
|Storage||-80°C; 1 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, and 0.5 mM DTT.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Osteoclast-stimulating factor-1 (OSTF1) is an intracellular protein produced by osteoclasts that indirectly induces osteoclast (OCL) formation and bone resorption. OSTF1 is highly expressed in OCL and contains structural domains such as SH3 and ankyrin repeats, which are potentially involved in protein-protein interactions. OSTF1 is ubiquitously expressed analogous to other regulators of OCL activity such as c-Src or transforming growth factor beta. Recombinant human OSTF1 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Reddy S.V.,et al.J. Cell. Physiol. 177:636-645(1998).
Suzuki Y.,et al.Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
Humphray S.J.,et al.Nature 429:369-374(2004).
Roodman G.D.,et al.Unpublished observations (NOV-2000).
Kurihara N.,et al.J. Biol. Chem. 276:41035-41039(2001).
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