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>   home   >   Products   >   Proteins   >   Human CellExp CD33 / SIGLEC-3, human recombinant protein   

Human CellExp CD33 / SIGLEC-3, human recombinant protein

CD33, SIGLEC3, gp67

Product info
Primary Accession P20138
Calculated MW This protein is fused with C-terminal 6×His tag, has a calculated MW of 27.6 kDa expressed. The predicted N-terminus is Asp 18. Protein migrates as 40-45 kDa in reduced SDS-PAGE.
Additional Info
Gene ID 945
Gene Symbol CD33
Other Names CD33, SIGLEC3, gp67
Gene Source Human
Source HEK293 cells
Assay&Purity SDS-PAGE; ≥95%
Assay2&Purity2 N/A;
Recombinant Yes
Results Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. The ED50 for this effect is typically 0.2-1.2 µg/ml.
Target/Specificity CD33/SIGLEC-3
Application Notes Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Format Lyophilized
Storage -20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Citations (0)

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Myeloid cell surface antigen CD33 also known as SIGLEC3, Siglecs (sialic acid binding Ig-like lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N--terminal Ig--like V--type domain, one Ig--like C2--type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 Domain (s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia.


Simmons D.,et al.J. Immunol. 141:2797-2800(1988).
Yousef G.M.,et al.Gene 286:259-270(2002).
Hernandez-Caselles T.,et al.J. Leukoc. Biol. 79:46-58(2006).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Grimwood J.,et al.Nature 428:529-535(2004).

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$ 195.00
$ 595.00
Cat# PBV10993r
(40 western blots)
Availability: 5-7days
Bulk Size
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