|Calculated MW||This protein is fused with C-terminal 6×His tag, has a calculated MW of 27.6 kDa expressed. The predicted N-terminus is Asp 18. Protein migrates as 40-45 kDa in reduced SDS-PAGE.|
|Other Names||CD33, SIGLEC3, gp67|
|Results||Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. The ED50 for this effect is typically 0.2-1.2 µg/ml.|
|Application Notes||Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.|
|Storage||-20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.|
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Myeloid cell surface antigen CD33 also known as SIGLEC3, Siglecs (sialic acid binding Ig-like lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N--terminal Ig--like V--type domain, one Ig--like C2--type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 Domain (s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia.
Simmons D.,et al.J. Immunol. 141:2797-2800(1988).
Yousef G.M.,et al.Gene 286:259-270(2002).
Hernandez-Caselles T.,et al.J. Leukoc. Biol. 79:46-58(2006).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Grimwood J.,et al.Nature 428:529-535(2004).
If you have any additional inquiries please email technical services at email@example.com.