Human CellExp CD33 / SIGLEC-3, human recombinant protein
CD33, SIGLEC3, gp67
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P20138 |
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Calculated MW | This protein is fused with C-terminal 6×His tag, has a calculated MW of 27.6 kDa expressed. The predicted N-terminus is Asp 18. Protein migrates as 40-45 kDa in reduced SDS-PAGE. |
Gene ID | 945 |
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Gene Symbol | CD33 |
Other Names | CD33, SIGLEC3, gp67 |
Gene Source | Human |
Source | HEK293 cells |
Assay&Purity | SDS-PAGE; ≥95% |
Assay2&Purity2 | N/A; |
Recombinant | Yes |
Results | Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. The ED50 for this effect is typically 0.2-1.2 µg/ml. |
Target/Specificity | CD33/SIGLEC-3 |
Application Notes | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
Format | Lyophilized |
Storage | -20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Myeloid cell surface antigen CD33 also known as SIGLEC3, Siglecs (sialic acid binding Ig-like lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N--terminal Ig--like V--type domain, one Ig--like C2--type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 Domain (s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia.
References
Simmons D.,et al.J. Immunol. 141:2797-2800(1988).
Yousef G.M.,et al.Gene 286:259-270(2002).
Hernandez-Caselles T.,et al.J. Leukoc. Biol. 79:46-58(2006).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Grimwood J.,et al.Nature 428:529-535(2004).
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