|Calculated MW||This protein is fused with C-terminal 6×his tag at C-terminus, has a calculated MW of 47.5 kDa expressed. The predicted N-terminus is Gly30. Protein migrates as 60-90 kDa in reduced SDS-PAGE resulting from glycosylation.|
|Other Names||CD36, SCARB3, GP3B, GP4, Platelet Glycoprotein 4|
|Results||Measured by its binding ability in a functional ELISA. Immobilized rhCD36 at 2 µg/mL (100 µL/well) can bind rhTSP2/His with a linear range of 0.01-1 µg/mL|
|Application Notes||Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 200 µg/ml. Solubilize for 30 to 60 min. at RT with occasional gentle mixing. Do not vortex. Carrier protein (0.1% HAS or BSA) is strongly recommended for further dilution and long term storage.|
|Storage||-20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.|
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Provided below are standard protocols that you may find useful for product applications.
CD36 (Cluster of Differentiation 36), also known as platelet membrane glycoprotein IV (GPIV), fatty acid translocase (FAT), thrombospondin receptor, collagen receptor, and scavenger receptor class B, member 3 (SRB3), is a member of the class B scavenger receptor family of cell surface proteins. The human CD36 gene encodes a single chain 472 amino acid residue protein containing both an N- and a C-terminal cytoplasmic tail and an extracellular loop.CD36 is found on platelets, erythrocytes, monocytes, differentiated adipocytes, mammary epithelial cells, spleen cells and some skin microdermal endothelial cells. CD36 is a multiligand pattern recognition receptor that interacts with a large number of structurally dissimilar ligands, including long chain fatty acid (LCFA), advanced glycation end products (AGE), thrombospondin-1, oxidized low-density lipoproteins (oxLDLs), high density lipoprotein (HDL), phosphatidylserine, apoptotic cells, beta-amyloid fibrils (fAβ), collagens I and IV, and Plasmodium falciparum infected erythrocytes. CD36 is required for the anti-angiogenic effects of thrombospondin1 in the corneal neovascularization assay. On binding a ligand the protein and ligand are internalized. This internalization is independent of macro pinocytosis and occurs by an actin dependent mechanism requiring the activation Src-family kinases, JNK and Rho-family GTPases. CD36 ligands have also been shown to promote sterile inflammation through assembly of a Toll-like receptor 4 and 6 heterodimer.
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Sugimoto Y.,et al.Submitted (AUG-1992) to the EMBL/GenBank/DDBJ databases.
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Wyler B.,et al.Thromb. Haemost. 70:500-505(1993).
Armesilla A.L.,et al.J. Biol. Chem. 269:18985-18991(1994).
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