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Human CellExp CD36, human recombinant protein

CD36, SCARB3, GP3B, GP4, Platelet Glycoprotein 4

Product info
Primary Accession P16671
Calculated MW This protein is fused with C-terminal 6×his tag at C-terminus, has a calculated MW of 47.5 kDa expressed. The predicted N-terminus is Gly30. Protein migrates as 60-90 kDa in reduced SDS-PAGE resulting from glycosylation.
Additional Info
Gene ID 948
Gene Symbol CD36
Other Names CD36, SCARB3, GP3B, GP4, Platelet Glycoprotein 4
Gene Source Human
Source HEK293 cells
Assay&Purity SDS-PAGE; ≥95%
Assay2&Purity2 N/A;
Recombinant Yes
Results Measured by its binding ability in a functional ELISA. Immobilized rhCD36 at 2 µg/mL (100 µL/well) can bind rhTSP2/His with a linear range of 0.01-1 µg/mL
Target/Specificity CD36
Application Notes Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 200 µg/ml. Solubilize for 30 to 60 min. at RT with occasional gentle mixing. Do not vortex. Carrier protein (0.1% HAS or BSA) is strongly recommended for further dilution and long term storage.
Format Lyophilized
Storage -20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Citations (0)

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CD36 (Cluster of Differentiation 36), also known as platelet membrane glycoprotein IV (GPIV), fatty acid translocase (FAT), thrombospondin receptor, collagen receptor, and scavenger receptor class B, member 3 (SRB3), is a member of the class B scavenger receptor family of cell surface proteins. The human CD36 gene encodes a single chain 472 amino acid residue protein containing both an N- and a C-terminal cytoplasmic tail and an extracellular loop.CD36 is found on platelets, erythrocytes, monocytes, differentiated adipocytes, mammary epithelial cells, spleen cells and some skin microdermal endothelial cells. CD36 is a multiligand pattern recognition receptor that interacts with a large number of structurally dissimilar ligands, including long chain fatty acid (LCFA), advanced glycation end products (AGE), thrombospondin-1, oxidized low-density lipoproteins (oxLDLs), high density lipoprotein (HDL), phosphatidylserine, apoptotic cells, beta-amyloid fibrils (fAβ), collagens I and IV, and Plasmodium falciparum infected erythrocytes. CD36 is required for the anti-angiogenic effects of thrombospondin1 in the corneal neovascularization assay. On binding a ligand the protein and ligand are internalized. This internalization is independent of macro pinocytosis and occurs by an actin dependent mechanism requiring the activation Src-family kinases, JNK and Rho-family GTPases. CD36 ligands have also been shown to promote sterile inflammation through assembly of a Toll-like receptor 4 and 6 heterodimer.


Oquendo P.,et al.Cell 58:95-101(1989).
Sugimoto Y.,et al.Submitted (AUG-1992) to the EMBL/GenBank/DDBJ databases.
Taylor K.T.,et al.Gene 133:205-212(1993).
Wyler B.,et al.Thromb. Haemost. 70:500-505(1993).
Armesilla A.L.,et al.J. Biol. Chem. 269:18985-18991(1994).

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$ 175.00
$ 575.00
Cat# PBV10994r
(40 western blots)
Availability: 5-7days
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