Human CellExp CD304 / NRP1 / Neurophilin-1, human recombinant protein
NRP1, Neuropilin-1, NRP, VEGF165R, CD304
|Calculated MW||This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 70.7 kDa. The predicted N-terminus is Phe 22. DTT-reduced Protein migrates as 85-90 kDa due to glycosylation.|
|Other Names||NRP1, Neuropilin-1, NRP, VEGF165R, CD304|
|Results||Measured by its binding ability in a functional ELISA. Immobilized rh NRP1 / Neuropilin‑1 / CD304 can bind rhVEGF165 with an apparent Kd < 1 nM.|
|Application Notes||Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.|
|Storage||-20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.|
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Neuropilin-1 (NRP1) is also known as vascular endothelial cell growth factor 165 receptor (VEGF165R), CD antigen CD304, which belongs to the neuropilin family. The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis. The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells.
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Gagnon M.L.,et al.Proc. Natl. Acad. Sci. U.S.A. 97:2573-2578(2000).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
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