|Calculated MW||This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 14.9 kDa. The predicted N-terminus is Gln 22. DTT-reduced Protein migrates as 19-23 kDa.|
|Other Names||LAIR2, CD306|
|Results||Measured by the ability of the immobilized protein to support the adhesion of HT-29 human colon adenocarcinoma cells. When 10000 cells/well are added to rhLAIR2 /CD306 coated plates (50 µg/ml with 100 µl/well), approximately >40% cells will adhere after 10 minutes at 37°C|
|Application Notes||Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.|
|Storage||-20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.|
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Provided below are standard protocols that you may find useful for product applications.
Leukocyte-associated immunoglobulin-like receptor 2 (LAIR2) is also known as CD antigen CD306. LAIR2 contains one Ig-like C2-type (immunoglobulin-like) domain. LAIR2 is a member of the immunoglobulin superfamily and was identified by its similarity to LAIR1, an inhibitory receptor present on mononuclear leukocytes. LAIR2 / CD306 is thought to be secreted and may help modulate mucosal tolerance.
Meyaard L.,et al.Immunity 7:283-290(1997).
Meyaard L.,et al.J. Immunol. 162:5800-5804(1999).
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