Human CellExp CD160/BY55, human recombinant protein
CD160, BY55, NK1, NK28
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | O95971 |
---|---|
Calculated MW | This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 15.6 kDa. The predicted N-terminus is Ile 27. DTT-reduced Protein migrates as 22-28 kDa due to glycosylation. |
Gene ID | 11126 |
---|---|
Gene Symbol | CD160 |
Other Names | CD160, BY55, NK1, NK28 |
Gene Source | Human |
Source | HEK293 cells |
Assay&Purity | SDS-PAGE; ≥95% |
Assay2&Purity2 | N/A; |
Recombinant | Yes |
Results | Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse HVEM Fc Chimera at 1 µg/ml, the concentration of rhCD160 that produces 50% of the optimal binding response is approximately 1.0‑16 ng/ml. |
Target/Specificity | CD160/BY55 |
Application Notes | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
Format | Lyophilized |
Storage | -20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
CD160 antigen also known as natural killer cell receptor BY55, is a 27 kDa glycoprotein, contains 1 Ig-like V-type (immunoglobulin-like) domain. Its expression is tightly associated with peripheral blood NK cells and CD8 T lymphocytes with cytolytic effector activity. CD160 is expressed at the cell surface as a tightly disulfide-linked multimer. In tissues, CD160 is expressed on all intestinal intraepithelial lymphocytes. CD160 shows a broad specificity for binding to both classical and non-classical MHC class I molecules. When expressed on vascular endothelial cells, CD160 propagates anti-angiogenic signals and promotes apoptosis.
References
Anumanthan A.,et al.J. Immunol. 161:2780-2790(1998).
Agrawal S.,et al.J. Immunol. 162:1223-1226(1999).
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.