|Calculated MW||This protein is fused with a 6×his tag at C-terminus and the protein has a calculated MW of 50.1 kDa. The predicted N-terminus is Asp68 & Tyr19. DTT-reduced protein migrates as 66-90 kDa in SDS-PAGE due to glycosylation.|
|Other Names||ANGPT2, AGPT2, ANG2, Angiopoietin-2|
|Results||Measured by its ability to activate Tie2 in human Tie2 transfected C6 rat glial cells. 0.2 µg/mL of rhANGPT2 significantly induces phosphorylation of human Tie2. Measured in a competitive binding assay. When Tie2 Fc Chimera is immobilized, rhAng2 inhibits binding of biotinylated rhAng-2.|
|Application Notes||Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.|
|Storage||-20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Angiopoietin-2, also known as ANGPT2, AGPT2, ANG2, and is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Ang2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel Palade bodies. Both Ang2 and the related Angiopoietin1 (Ang1) are ligands for the receptor tyrosine kinase Tie 2. Ang2 functions as a proangiogenic factor, although it can also induce EC death and vessel regression. Upon its release from quiescent EC, it regulates vascular remodeling by promoting EC survival, proliferation, and migration and destabilizing the interaction between EC and perivascular cells. Ang2 is required for postnatal vascular remodeling, and it cooperates with Ang1 during lymphatic vessel development. It mediates the upregulation of ICAM1 and VCAM1 on EC, which facilitates the adhesion of leukocytes during inflammation. Ang2 competitively inhibit Ang1-induced endothelial cell responses mediated by Tie2, and reduces vascular integrity. But the role of Ang2 is controversial since the opposite outcomes have been reported in other studies. Over-expression of Ang2 disrupts the vascular remodeling, induces endothelial cell apoptosis, and may play an important regulating role in tumor angiogenesis. Ang2 also promotes the neuronal differentiation and migration of sub ventricular zone progenitor cells.
Maisonpierre P.C.,et al.Science 277:55-60(1997).
Tanaka S.,et al.J. Clin. Invest. 103:341-345(1999).
Kim I.,et al.J. Biol. Chem. 275:18550-18556(2000).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Nusbaum C.,et al.Nature 439:331-335(2006).
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.