|Calculated MW||This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 69.5 kDa. The predicted N-terminus is Leu 25. DTT-reduced Protein migrates as 110-115 kDa due to glycosylation.|
|Other Names||EGFR, ERBB, ERBB1, HER1, PIG61, mENA|
|Results||Measured by its binding ability in a functional ELISA. Immobilized human EGF at 10 µg/ml can bind human EGFR with a linear range of 0.5–750 ng/ml.|
|Application Notes||Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.|
|Storage||-20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.
Ullrich A.,et al.Nature 309:418-425(1984).
Ilekis J.V.,et al.Mol. Reprod. Dev. 41:149-156(1995).
Reiter J.L.,et al.Nucleic Acids Res. 24:4050-4056(1996).
Ilekis J.V.,et al.Gynecol. Oncol. 65:36-41(1997).
Reiter J.L.,et al.Genomics 71:1-20(2001).
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