|Calculated MW||This protein has a C-terminal 6×His tag and has a calculated MW of 70 kDa. The predicted N-terminus is Leu 25. In DTT-reduced SDS-PAGE, protein migrates as 110-115 kDa.|
|Other Names||EGFR, ERBB, ERBB1, HER1, PIG61, mENA|
|Results||Measured by its binding ability in a functional ELISA. Immobilized mouse EGF at 10 µg/ml can bind mouse EGFR with a linear range of 2.5–1000 ng/ml.|
|Application Notes||Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.|
|Storage||-20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.|
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Provided below are standard protocols that you may find useful for product applications.
The epidermal growth factor receptor (EGFR; ErbB-1; HER1) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.
Avivi A.,et al.Oncogene 7:1957-1962(1992).
Paria B.C.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:55-59(1993).
Hibbs M.L.,et al.Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases.
Luetteke N.C.,et al.Genes Dev. 8:399-413(1994).
Avivi A.,et al.Oncogene 6:673-676(1991).
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