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BRD1 bromodomain (556-688 aa) (His-Tag), human recombinant protein
Bromodomain containing 1, DKFZp686F0325, BR140-like protein, BRL, BRPF1, BRPF2, Bromodomain & PHD fi
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | O95696 |
|---|---|
| Concentration | 0.5 |
| Calculated MW | 17.8 kDa (156 aa, 556-688 aa + His Tag), confirmed by MALDI-TOF. |
| Gene ID | 23774 |
|---|---|
| Gene Symbol | BRD1 |
| Other Names | Bromodomain containing 1, DKFZp686F0325, BR140-like protein, BRL, BRPF1, BRPF2, Bromodomain & PHD finger-containing protein 2 |
| Gene Source | Human |
| Source | E. coli |
| Assay&Purity | SDS-PAGE; ≥95% |
| Assay2&Purity2 | N/A; |
| Recombinant | Yes |
| Sequence | MGSSHHHHHH SSGLVPRGSH MGSEQVAMEL RLTPLTVLLR SVLDQLQDKD PARIFAQPVS LKEVPDYLDH IKHPMDFATM RKRLEAQGYK NLHEFEEDFD LIIDNCMKYN ARDTVFYRAA VRLRDQGGVV LRQARREVDS IGLEEASGMH LPERPA |
| Target/Specificity | BRD1 |
| Format | Liquid |
| Storage | -80°C; 0.5 mg/ml in Phosphate buffer saline (pH 7.4) containing 10% glycerol and 1mM DTT. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. BRD1 is a bromodomain containing protein that has been identified as a susceptibility gene in neurological disorders, such as schizophrenia and bipolar affective disorder. This protein can be used for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling. Recombinant human BRD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
References
McCullagh P.,et al.Oncogene 18:7442-7452(1999).
Collins J.E.,et al.Genome Biol. 5:R84.1-R84.11(2004).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Dunham I.,et al.Nature 402:489-495(1999).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
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