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Human CellExp Kallikrein-1, human recombinant protein
KLK1, Kallikrein-1, KLKR, Klk6, hK1
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P06870 |
|---|---|
| Calculated MW | This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 27.9 kDa. The predicted N-terminus is Pro 19. DTT-reduced Protein migrates as 40-45 kDa due to glycosylation. |
| Gene ID | 3816 |
|---|---|
| Gene Symbol | KLK1 |
| Other Names | KLK1, Kallikrein-1, KLKR, Klk6, hK1 |
| Gene Source | Human |
| Source | HEK293 cells |
| Assay&Purity | SDS-PAGE; ≥95% |
| Assay2&Purity2 | N/A; |
| Recombinant | Yes |
| Results | Measured by its ability to cleave a fluorogenic peptide substrate Pro – Phe – Arg – 7 – amido – 4 - methylcoumarin (PFR-AMC). The specific activity is >1200 pmol/min/ µg. |
| Target/Specificity | Kallikrein-1 |
| Application Notes | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| Format | Lyophilized |
| Storage | -20°C; Lyophilized from 0.22 µm filtered solution in 50 mM Tris, 2 mM CaCl2, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Kallikrein-1 (KLK1) belongs to the peptidase S1 family and Kallikrein subfamily. KLK1 contains one peptidase S1 domain. KLK-1 preferential cleavage of Arg-|-Xaa bonds in small molecule substrates and also has highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa. Glandular Kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
References
Fukushima D.,et al.Biochemistry 24:8037-8043(1985).
Evans B.A.,et al.Biochemistry 27:3124-3129(1988).
Angermann A.,et al.Biochem. J. 262:787-793(1989).
Chen L.-M.,et al.Braz. J. Med. Biol. Res. 27:1829-1838(1994).
Yousef G.M.,et al.Biochem. Biophys. Res. Commun. 276:125-133(2000).
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