|Calculated MW||This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 45.2 kDa. The predicted N-terminus is Gln 20. DTT-reduced Protein migrates as 45-55 kDa.|
|Other Names||SERPINF1, Serpin F1, PEDF, PIG35, EPC-1|
|Results||Measured by its ability to enhance the adhesion of Saos‑2 human osteosarcoma cells to bovine Collagen I coated plate. The ED50 for this effect is typically 0.5-1.6 µg/ml.|
|Application Notes||Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.|
|Storage||-20°C; Lyophilized from 0.22 µm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.|
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Serpin F1 (SERPINF1) is also known as Pigment epithelium-derived factor (PEDF), Cell proliferation-inducing gene 35 protein (PIG35). Serpin F1 belongs to the serpin family. Serpin F1 is expressed in quiescent cells. PEDF has a variety of functions including antiangiogenic, antitumorigenic, and neurotrophic properties. Endothelial cell migration is inhibited by SERPINF1/ PEDF. PEDF / SERPINF1 suppresses retinal neovascularization and endothelial cell proliferation. PEDF is also responsible for apoptosis of endothelial cells either through the p38 MAPK pathway or through the FAS/FASL pathway. PEDF also displays neurotrophic functions.
Steele F.R.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:1526-1530(1993).
Tombran-Tink J.,et al.Mol. Vis. 2:11-11(1996).
Yin B.,et al.Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Kim J.W.,et al.Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases.
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