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Human CellExp SERPIN A3, human recombinant protein


Product info
Primary Accession P01011
Calculated MW This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 46 kDa. The predicted N-terminus is His 23. DTT-reduced Protein migrates as 55-70 kDa due to glycosylation.
Additional Info
Gene ID 12
Gene Symbol SERPINA3
Other Names SERPINA3, ACT, AACT, GIG24, GIG25
Gene Source Human
Source HEK293 cells
Assay&Purity SDS-PAGE; ≥95%
Assay2&Purity2 N/A;
Recombinant Yes
Results Measured by its ability to inhibit chymotrypsin cleavage of a fluorogenic peptide substrate MCA-RPKPVE-Nval-WRK (Dnp)-NH2. The IC50 value is <6 nM.
Target/Specificity SERPIN A3
Application Notes Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Format Lyophilized
Storage -20°C; Lyophilized from 0.22 µm filtered solution in 20 mM HEPES, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Citations (0)

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Serpin A3 is also known as Alpha-1-antichymotrypsin (ACT or AACT), Cell growth-inhibiting gene 24/25 protein, which belongs to the serpin family. Serpin A3 can bind DNA. Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.


Chandra T.,et al.Biochemistry 22:5055-5061(1983).
Poller W.,et al.Genomics 17:740-743(1993).
Kim J.W.,et al.Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.

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$ 215.00
Cat# PBV11041r
(40 western blots)
Availability: 5-7days
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