|Calculated MW||This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 46 kDa. The predicted N-terminus is His 23. DTT-reduced Protein migrates as 55-70 kDa due to glycosylation.|
|Other Names||SERPINA3, ACT, AACT, GIG24, GIG25|
|Results||Measured by its ability to inhibit chymotrypsin cleavage of a fluorogenic peptide substrate MCA-RPKPVE-Nval-WRK (Dnp)-NH2. The IC50 value is <6 nM.|
|Application Notes||Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.|
|Storage||-20°C; Lyophilized from 0.22 µm filtered solution in 20 mM HEPES, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Serpin A3 is also known as Alpha-1-antichymotrypsin (ACT or AACT), Cell growth-inhibiting gene 24/25 protein, which belongs to the serpin family. Serpin A3 can bind DNA. Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.
Chandra T.,et al.Biochemistry 22:5055-5061(1983).
Poller W.,et al.Genomics 17:740-743(1993).
Kim J.W.,et al.Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
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