|Calculated MW||This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 25.6 kDa. The predicted N-terminus is Phe 19. DTT-reduced Protein migrates as 40-50 kDa due to glycosylation.|
|Other Names||PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1|
|Results||Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse PDL1 at 1 µg/ml (100 µl/well) can bind biotinylated Mouse PDCD1 / PD1 with a linear range of 16 - 500 ng/ml.|
|Target/Specificity||PD-L1 /CD274 /B7-H1|
|Application Notes||Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.|
|Storage||-20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.|
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Provided below are standard protocols that you may find useful for product applications.
Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.
Freeman G.J.,et al.J. Exp. Med. 192:1027-1034(2000).
Tamura H.,et al.Blood 97:1809-1816(2001).
Latchman Y.,et al.Nat. Immunol. 2:261-268(2001).
Wang S.,et al.J. Exp. Med. 197:1083-1091(2003).
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