Human CellExp PD-L1 /CD274 /B7-H1, mouse recombinant protein
PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q9EP73 |
---|---|
Calculated MW | This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 25.6 kDa. The predicted N-terminus is Phe 19. DTT-reduced Protein migrates as 40-50 kDa due to glycosylation. |
Gene ID | 60533 |
---|---|
Gene Symbol | CD274 |
Other Names | PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1 |
Gene Source | Mouse |
Source | HEK293 cells |
Assay&Purity | SDS-PAGE; ≥95% |
Assay2&Purity2 | N/A; |
Recombinant | Yes |
Results | Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse PDL1 at 1 µg/ml (100 µl/well) can bind biotinylated Mouse PDCD1 / PD1 with a linear range of 16 - 500 ng/ml. |
Target/Specificity | PD-L1 /CD274 /B7-H1 |
Application Notes | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
Format | Lyophilized |
Storage | -20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.
References
Freeman G.J.,et al.J. Exp. Med. 192:1027-1034(2000).
Tamura H.,et al.Blood 97:1809-1816(2001).
Latchman Y.,et al.Nat. Immunol. 2:261-268(2001).
Wang S.,et al.J. Exp. Med. 197:1083-1091(2003).
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.