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Human CellExp PD-L1 /CD274 /B7-H1, human recombinant protein
PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q9NZQ7 |
|---|---|
| Calculated MW | This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 26 kDa. The predicted N-terminus is Phe 19. DTT-reduced Protein migrates as 35-42 kDa due to glycosylation. |
| Gene ID | 29126 |
|---|---|
| Gene Symbol | CD274 |
| Other Names | PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1 |
| Gene Source | Human |
| Source | HEK293 cells |
| Assay&Purity | SDS-PAGE; ≥95% |
| Assay2&Purity2 | N/A; |
| Recombinant | Yes |
| Results | Measured by its binding ability in a functional ELISA. Immobilized rh PD-L1 / B7-H1 at 2 µg/ml (100 µl/well) can bind rhPD1/PDCD1 Fc Chimera with a linear range of 1-10 µg/ml. Measured by its ability to inhibit antiCD3 antibody induced IL2 secretion in human T lymphocytes. The ED50 for this effect is typically 0.6-5 µg/ml. |
| Target/Specificity | PD-L1 /CD274 /B7-H1 |
| Application Notes | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| Format | Lyophilized |
| Storage | -20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.
References
Dong H.,et al.Nat. Med. 5:1365-1369(1999).
Freeman G.J.,et al.J. Exp. Med. 192:1027-1034(2000).
He X.-H.,et al.Acta Pharmacol. Sin. 26:462-468(2005).
Chi X.-Y.,et al.Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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