Human CellExp TIMP1, human recombinant protein
TIMP1, TIMP-1, CLGI, EPA, EPO, FLJ90373, HCI, TIMP, TIMP metallopeptidase inhibitor 1
|Calculated MW||This protein contains C-terminal polyhistidine tag and has a calculated MW of 24 kDa. DTT-reduced protein migrates as 28-32 kDa polypeptide in SDS-PAGE.|
|Other Names||TIMP1, TIMP-1, CLGI, EPA, EPO, FLJ90373, HCI, TIMP, TIMP metallopeptidase inhibitor 1|
|Results||Measured by its ability to inhibit human MMP2 cleavage of a fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2.The IC50 value is approximately 2.5 nM.|
|Application Notes||Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.|
|Storage||-20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.|
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TIMP metallopeptidase inhibitor 1, also known as TIMP1, a tissue inhibitor of metalloproteinases, is a glycoprotein that is expressed from the several tissues of organisms. This protein a member of the TIMP family. There are four members of the family, TIMP1, TIMP2, TIMP3 and TIMP4. TIMP1 is a glycoprotein with a molecular mass of 28 kDa produced by a wide range of cell types. The glycoprotein is a natural inhibitor of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction.
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Gasson J.C.,et al.Nature 315:768-771(1985).
Carmichael D.F.,et al.Proc. Natl. Acad. Sci. U.S.A. 83:2407-2411(1986).
Kaczorek M.,et al.Biotechnology (N.Y.) 5:595-598(1987).
Rapp G.,et al.DNA Cell Biol. 9:479-485(1990).
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