Human CellExp CNTN2/Contactin-2, human recombinant protein
CNTN2, CNTN-2, AXT, DKFZp781D102, FLJ37193, FLJ42746, MGC157722, TAG-1, TAG1, TAX, TAX1, TAX-1, Cont
|Calculated MW||This protein is fused with 6×his tag at the C-terminus and has a calculated MW of 109 kDa expressed. The predicted N-terminus is Ser 31. Protein migrates as 140 kDa in reduced SDS-PAGE resulting from glycosylation.|
|Other Names||CNTN2, CNTN-2, AXT, DKFZp781D102, FLJ37193, FLJ42746, MGC157722, TAG-1, TAG1, TAX, TAX1, TAX-1, Contactin-2|
|Results||Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Optimal neurite outgrowth was observed when neurons were plated on 96-well culture plates that had been precoated with 50 µl/well of the rhContactin2 solution at 8-30 µg/ml.|
|Application Notes||Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.|
|Storage||-20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Contactin-2 also known as CNTN2, TAX1 (transiently-expressed axonal glycoprotein), TAG1 (transient axonal glycoprotein), and axonin-1, and is a member of the immunoglobulin superfamily. CNTN2 consists of six Ig-like domains and four fibronectin type III domains, and is anchored to the membrane by glycosylphosphatidylinositol (GPI), whereas the soluble form can be released by a GPI-specific phospholipase. As a neural cell adhesion molecule expressed by a subset of neuronal populations in the developing CNS and PNS, CNTN2 mediates cell-cell interactions either via homophilic, or heterophilic contacts with various adhesion molecules including NgCAM, NrCAM, NCAM and neurocan. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. It may also be involved in glial tumorigenesis and may provide a potential target for therapeutic intervention.
Hasler T.H.,et al.Eur. J. Biochem. 211:329-339(1993).
Tsiotra C.P.,et al.Genomics 18:562-567(1993).
Kozlov S.V.,et al.Genomics 30:141-148(1995).
Gregory S.G.,et al.Nature 441:315-321(2006).
Tsiotra C.P.,et al.Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.