|Calculated MW||This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 30 kDa. The predicted N-terminus is Asp 29. DTT-reduced Protein migrates as 41-45 kDa due to glycosylation.|
|Other Names||TFPI, LACI, TFPI1, EPI, TFI|
|Results||Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca - RPKPVE - Nval – WRK (Dnp) - NH2. The IC50 value is < 0.35 nM, as measured in 100 µl reaction mixture containing 1.25 ng trypsin, 10 µM substrate, 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% Brij-35 (w/v), pH 7.5.|
|Application Notes||Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.|
|Storage||-20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Tissue factor pathway inhibitor (TFPI) also known as Extrinsic pathway inhibitor (EPI), Lipoprotein - associated coagulation inhibitor (LACI), is a plasma proteinase inhibitor synthesized by vascular endothelial cells and part of it is associated with glycosaminoglycans of these cells. TFPI is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa). TFPI is a secreted protein with a Nterminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a Cterminal basic region. The first K domain inhibits coagulation factor VIIa complexed to tissue factor (TF); The second K domain inhibits factor Xa; The third K domain binds to heparin; The Cterminal basic region may have several functions. For example, it plays an important role in binding of TFPI to cell surfaces.
Wun T.-C.,et al.J. Biol. Chem. 263:6001-6004(1988).
Girard T.J.,et al.Thromb. Res. 55:37-50(1989).
van der Logt C.P.E.,et al.Biochemistry 30:1571-1577(1991).
Girard T.J.,et al.J. Biol. Chem. 266:5036-5041(1991).
Chang J.-Y.,et al.Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.