|Calculated MW||This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 33.4 kDa. The predicted N-terminus is Ser 32. DTT-reduced Protein migrates as 38 kDa due to glycosylation.|
|Other Names||SFRP1, FRP, FRP1, SARP2, FrzA|
|Results||Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED50 for this effect is typically 2.5 - 10 µg/ml.|
|Application Notes||Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.|
|Storage||-20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.|
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Provided below are standard protocols that you may find useful for product applications.
Secreted frizzled-related protein 1 (SFRP1) also known as Secreted apoptosis-related protein 2 (SARP2), FRP, FRP1, is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The Secreted frizzled-related protein (SFRP) family consists of five secreted glycoproteins in humans (SFRP1, SFRP2, SFRP3, SFRP4, SFRP5) that act as extracellular signaling ligands. SFRP1 / SARP-2 is widely expressed and absent from lung, liver and peripheral blood leukocytes, and with the highest levels in heart and fetal kidney. SARP2 / SFRP-1 is a 35 kDa prototypical member of the SFRP family. SARP2 / FRP-1 acts as a biphasic modulator of Wnt signaling, counteracting Wnt-induced effects at high concentrations and promoting them at lower concentrations.
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Melkonyan H.S.,et al.Proc. Natl. Acad. Sci. U.S.A. 94:13636-13641(1997).
Zhou Z.,et al.Int. J. Cancer 78:95-99(1998).
Chong J.M.,et al.J. Biol. Chem. 277:5134-5144(2002).
Bafico A.,et al.J. Biol. Chem. 274:16180-16187(1999).
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