Human CellExp GFRA1 /GDNFRA, human recombinant protein
GFRA1, GDNFRA, RETL1, TRNR1, GDNFR, GFR-ALPHA-1, RET1L
|Calculated MW||This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 45.5 kDa. The predicted N-terminus is Asp 25. DTT-reduced Protein migrates as 55-60 kDa due to glycosylation.|
|Other Names||GFRA1, GDNFRA, RETL1, TRNR1, GDNFR, GFR-ALPHA-1, RET1L|
|Results||Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human GDNF at 1 µg/mL binds rhGFRA1 with an apparent KD <10 nM.|
|Application Notes||Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.|
|Storage||-20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
GDNF family receptor alpha-1 (GFRA1) also known as RET ligand 1 (RETL1), TGF-beta-related neurotrophic factor receptor 1 (TRNR1), is a novel glycosylphosphatidylinositol (GPI)-linked cell surface receptor, and belongs to the glial cell line-derived neurotrophic factor (GDNF) receptor subfamily. The GDNF family ligands comprises GDNF, Neurturin (NTN), Artemin, and Persephin. GDNF and NTN are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GFRA1 mediates the association with and activation of the RET tyrosine kinase receptor (RTK), and subsequently initiates the RET signaling pathway. GFR alpha-mediated signaling plays an important role in the survival, differentiation, and migration of central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system, as well as the inflammatory response in some carcinomas.
Jing S.,et al.Cell 85:1113-1124(1996).
Sanicola M.,et al.Proc. Natl. Acad. Sci. U.S.A. 94:6238-6243(1997).
Angrist M.,et al.Genomics 48:354-362(1998).
Shefelbine S.E.,et al.Hum. Genet. 102:474-478(1998).
Hishiki T.,et al.Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.