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Human CellExp KITLG /SCF, mouse recombinant protein
KITLG, FPH2, KL-1, Kitl, MGF, SCF, SF, SHEP7, KL.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P20826 |
|---|---|
| Calculated MW | This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 19.1 kDa. The predicted N-terminus is Lys 26. DTT-reduced Protein migrates as 19-30 kDa due to different glycosylation. |
| Gene ID | 17311 |
|---|---|
| Gene Symbol | KITLG |
| Other Names | KITLG, FPH2, KL-1, Kitl, MGF, SCF, SF, SHEP7, KL. |
| Gene Source | Mouse |
| Source | HEK293 cells |
| Assay&Purity | SDS-PAGE; ≥98% |
| Assay2&Purity2 | N/A; |
| Recombinant | Yes |
| Results | Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5 - 8 ng/ml. |
| Target/Specificity | KITLG /SCF |
| Application Notes | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| Format | Lyophilized |
| Storage | -20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Kit ligand (KITLG) also known as stem cell factor (SCF), mast cell growth factor (MGF), steel factor (SF), which belongs to the SCF family, and is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein. KITLG is the ligand for the receptor-type protein-tyrosine kinase KIT. SCF / MGF plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG / SCF binding can activate several signaling pathways. KITLG / SF Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG / SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG / SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG / SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1, 4, 5 - triphosphate. KITLG / SCF acts synergistically with other cytokines, probably interleukins.
References
Anderson D.M.,et al.Cell 63:235-243(1990).
Huang E.J.,et al.Mol. Biol. Cell 3:349-362(1992).
Flanagan J.G.,et al.Cell 64:1025-1035(1991).
Brannan C.I.,et al.Genes Dev. 6:1832-1842(1992).
Bedell M.A.,et al.Genetics 142:927-934(1996).
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