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Human CellExp IL-21, human recombinant protein
Human IL-21, Interleukin-21 Protein
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q9HBE4 |
|---|---|
| Calculated MW | This protein contains no "tag", has a calculated MW of 15.6 kDa. The predicted N-terminus is Gln 23. DTT-reduced Protein migrates as 20 kDa due to glycosylation. |
| Gene ID | 59067 |
|---|---|
| Gene Symbol | IL21 |
| Other Names | Human IL-21, Interleukin-21 Protein |
| Gene Source | Human |
| Source | HEK293 cells |
| Assay&Purity | SDS-PAGE; ≥92% |
| Assay2&Purity2 | N/A; |
| Recombinant | Yes |
| Results | The bio-activity was determined by its ability to induce IFN-γ production by NK-92 cells. The ED50 < 3 ng/ml, corresponding to a specific activity of >3X105 Unit/mg. |
| Target/Specificity | IL-21 |
| Application Notes | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| Format | Lyophilized |
| Storage | -20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Interleukin-21 (IL-21) is a secreted protein which belongs to the IL-15 / IL-21 family. Interleukin-21 / IL-21 belongs to a family of cytokines that bind to a composite receptor consisting of a private receptor (IL21R) and the common cytokine receptor gamma chain (gamma(C)). Interleukin-21 / IL-21 impacts a number of cell types, including CD8+ memory T cells, NK cells and subsets of CD4 memory T cells. The IL-21R is widely distributed on lympho-haematopoietic cells. IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen-specific responses of immune cells. IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the γ chain (γc) found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3.
References
Parrish-Novak J.,et al.Nature 408:57-63(2000).
Rahman M.,et al.FEBS Lett. 581:4001-4009(2007).
Hillier L.W.,et al.Nature 434:724-731(2005).
Strengell M.,et al.J. Leukoc. Biol. 76:416-422(2004).
Sivakumar P.V.,et al.Immunology 112:177-182(2004).
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