|Calculated MW||This protein contains no "tag", has a calculated MW of 15.6 kDa. The predicted N-terminus is Gln 23. DTT-reduced Protein migrates as 20 kDa due to glycosylation.|
|Other Names||Human IL-21, Interleukin-21 Protein|
|Results||The bio-activity was determined by its ability to induce IFN-γ production by NK-92 cells. The ED50 < 3 ng/ml, corresponding to a specific activity of >3X105 Unit/mg.|
|Application Notes||Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.|
|Storage||-20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.|
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Provided below are standard protocols that you may find useful for product applications.
Interleukin-21 (IL-21) is a secreted protein which belongs to the IL-15 / IL-21 family. Interleukin-21 / IL-21 belongs to a family of cytokines that bind to a composite receptor consisting of a private receptor (IL21R) and the common cytokine receptor gamma chain (gamma(C)). Interleukin-21 / IL-21 impacts a number of cell types, including CD8+ memory T cells, NK cells and subsets of CD4 memory T cells. The IL-21R is widely distributed on lympho-haematopoietic cells. IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen-specific responses of immune cells. IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the γ chain (γc) found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3.
Parrish-Novak J.,et al.Nature 408:57-63(2000).
Rahman M.,et al.FEBS Lett. 581:4001-4009(2007).
Hillier L.W.,et al.Nature 434:724-731(2005).
Strengell M.,et al.J. Leukoc. Biol. 76:416-422(2004).
Sivakumar P.V.,et al.Immunology 112:177-182(2004).
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