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Human CellExp IGF1R/CD221, human recombinant protein
IGF-1R, IGF1R, CD221, IGFIR, IGFⅠR, IGFR, JTK13, MGC142170, MGC142172, MGC18216, Insulin-like Growth
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P08069 |
|---|---|
| Calculated MW | This protein contains C-terminal polyhistidine tag and has a calculated MW of 104 kDa (single chain), 80 kDa (α subunit) and 23 kDa (β subunit). DTT-reduced protein migrates as 120 kDa, 80 kDa and 35 kDa polypeptide in SDS-PAGE. |
| Gene ID | 3480 |
|---|---|
| Gene Symbol | IGF1R |
| Other Names | IGF-1R, IGF1R, CD221, IGFIR, IGFⅠR, IGFR, JTK13, MGC142170, MGC142172, MGC18216, Insulin-like Growth Factor 1 (IGF-1) Receptor |
| Gene Source | Human |
| Source | HEK293 cells |
| Assay&Purity | SDS-PAGE; ≥95% |
| Assay2&Purity2 | N/A; |
| Recombinant | Yes |
| Results | Measured by its ability to bind human IGF-I in a functional ELISA. |
| Target/Specificity | IGF1R/CD221 |
| Application Notes | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| Format | Lyophilized |
| Storage | -20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
The Insulin-like Growth Factor 1 Receptor （IGF1R）is also known as CD221, JTK13, and is a transmembrane receptor that is activated by IGF-1 and by the related growth factor IGF-2. It belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. IGF1R is made up of two alpha subunits and two beta subunits. Both the α and β subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds to form a functional transmembrane αβ chain. The α chains are located extracellularly while the β subunit spans the membrane and are responsible for intracellular signal transduction upon ligand stimulation. IGF1R have a binding site for ATP, which is used to provide the phosphates for autophosphorylation. There is a 60% homology between IGF1R and the insulin receptor. In response to ligand binding, the α chains induce the tyrosine autophosphorylation of the β chains. This event triggers a cascade of intracellular signaling that, while somewhat cell type specific, often promotes cell survival and cell proliferation.
References
Ullrich A.,et al.EMBO J. 5:2503-2512(1986).
Abbot A.M.,et al.J. Biol. Chem. 267:10759-10763(1992).
Nagase T.,et al.Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases.
Zody M.C.,et al.Nature 440:671-675(2006).
Cooke D.W.,et al.Biochem. Biophys. Res. Commun. 177:1113-1120(1991).
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