Human CellExp IGF1R/CD221, human recombinant protein
IGF-1R, IGF1R, CD221, IGFIR, IGFⅠR, IGFR, JTK13, MGC142170, MGC142172, MGC18216, Insulin-like Growth
|Calculated MW||This protein contains C-terminal polyhistidine tag and has a calculated MW of 104 kDa (single chain), 80 kDa (α subunit) and 23 kDa (β subunit). DTT-reduced protein migrates as 120 kDa, 80 kDa and 35 kDa polypeptide in SDS-PAGE.|
|Other Names||IGF-1R, IGF1R, CD221, IGFIR, IGFⅠR, IGFR, JTK13, MGC142170, MGC142172, MGC18216, Insulin-like Growth Factor 1 (IGF-1) Receptor|
|Results||Measured by its ability to bind human IGF-I in a functional ELISA.|
|Application Notes||Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.|
|Storage||-20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.|
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Provided below are standard protocols that you may find useful for product applications.
The Insulin-like Growth Factor 1 Receptor （IGF1R）is also known as CD221, JTK13, and is a transmembrane receptor that is activated by IGF-1 and by the related growth factor IGF-2. It belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. IGF1R is made up of two alpha subunits and two beta subunits. Both the α and β subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds to form a functional transmembrane αβ chain. The α chains are located extracellularly while the β subunit spans the membrane and are responsible for intracellular signal transduction upon ligand stimulation. IGF1R have a binding site for ATP, which is used to provide the phosphates for autophosphorylation. There is a 60% homology between IGF1R and the insulin receptor. In response to ligand binding, the α chains induce the tyrosine autophosphorylation of the β chains. This event triggers a cascade of intracellular signaling that, while somewhat cell type specific, often promotes cell survival and cell proliferation.
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Nagase T.,et al.Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases.
Zody M.C.,et al.Nature 440:671-675(2006).
Cooke D.W.,et al.Biochem. Biophys. Res. Commun. 177:1113-1120(1991).
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