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Human CellExp KLK-8 / Kallikrein-8, human recombinant protein
KLK8, Kallikrein-8, Neuropsin, Ovasin, NRPN, PRSS19, TADG14, NP, hK8
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | O60259 |
|---|---|
| Calculated MW | This protein is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 25.8 kDa. The predicted N-terminus is Gln 29. DTT-reduced Protein migrates as 38 kDa in SDS-PAGE due to glycosylation. |
| Gene ID | 11202 |
|---|---|
| Gene Symbol | KLK8 |
| Other Names | KLK8, Kallikrein-8, Neuropsin, Ovasin, NRPN, PRSS19, TADG14, NP, hK8 |
| Gene Source | Human |
| Source | HEK293 cells |
| Assay&Purity | SDS-PAGE; ≥95% |
| Assay2&Purity2 | N/A; |
| Recombinant | Yes |
| Results | >500 pmoles / min / µg |
| Sequence | Gln 29 – Gly 260 |
| Target/Specificity | KLK-8 / Kallikrein-8 |
| Application Notes | Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex. |
| Format | Lyophilized |
| Storage | -20°C; Lyophilized from 0.22 µm filtered solution in 50 mM Tris, 150 mM NaCl, pH 8.0. Normally Mannitol or Trehalose are added as protectants before lyophilization. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Kallikrein-8 (KLK8) is also known as Neuropsin (NP or NRPN), Ovasin, Serine protease 19 (PRSS19), Tumor-associated differentially expressed gene 14 protein (TADG-14), which belongs to the peptidase S1 family and Kallikrein subfamily. KLK8 contains 1 peptidase S1 domain. KLK8 is pH dependence protein and the optimum pH is 8.5, and the protein is active from pH 7-10. KLK8 is expressed at high levels in serum, ascites fluid and tumor cytosol of advanced stage ovarian cancer patients and may serve as a marker of ovarian cancer. KLK8 cleavage of amide substrates following the basic amino acids Arg or Lys at the P1 position, with a preference for Arg over Lys, and the catalytic activity of KLK8 is inhibited by a range of serine protease inhibitors including antipain, aprotinin, leupeptin, benzamidine and soybean trypsin inhibitor.
References
Yoshida S.,et al.Gene 213:9-16(1998).
Underwood L.J.,et al.Cancer Res. 59:4435-4439(1999).
Mitsui S.,et al.Eur. J. Biochem. 260:627-634(1999).
Gan L.,et al.Gene 257:119-130(2000).
Magklara A.,et al.Clin. Cancer Res. 7:806-811(2001).
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