Human CellExp ENPP-2 / Autotaxin, human recombinant protein
ENPP2, E-NPP 2, Autotaxin, LysoPLD, ATX, PDNP2, ATX-X, NPP2, PD-IALPHA
|Calculated MW||This protein is fused with polyhistidine tag at the N-terminus, has a calculated MW of 94.5 kDa. The predicted N-terminus is Asp 49. DTT-reduced Protein migrates as 120-130 kDa due to glycosylation.|
|Other Names||ENPP2, E-NPP 2, Autotaxin, LysoPLD, ATX, PDNP2, ATX-X, NPP2, PD-IALPHA|
|Results||>8000 pmol/min/ µg|
|Target/Specificity||ENPP-2 / Autotaxin|
|Application Notes||Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.|
|Storage||-20°C; Lyophilized from 0.22 µm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.|
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Provided below are standard protocols that you may find useful for product applications.
Autotaxin also known as ectonucleotide pyrophosphatase / phosphodiesterase family member 2 (E-NPP 2 or ENPP2), Extracellular lysophospholipase D (LysoPLD), Autotaxin (ATX or ATX-X), which belongs to the nucleotide pyrophosphatase / phosphodiesterase family. ENPP2 contains two SMB (somatomedin-B) domains. ENPP2 hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. ENPP2 can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. ENPP2 stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein.
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Kawagoe H.,et al.Genomics 30:380-384(1995).
Lee H.Y.,et al.Biochem. Biophys. Res. Commun. 218:714-719(1996).
van Meeteren L.A.,et al.J. Biol. Chem. 280:21155-21161(2005).
Giganti A.,et al.J. Biol. Chem. 283:7776-7789(2008).
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