Active Caspase-1, rat recombinant protein
Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, IL-1BC, p45.
|Calculated MW||~ 34.6 KDa|
|Other Names||Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, IL-1BC, p45.|
|Application Notes||Reconstitute to 1 unit per µl in PBS containing 15% glycerol.|
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Provided below are standard protocols that you may find useful for product applications.
Caspase-1 (also known as ICE) is a prototypical member of the caspase-family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa proenzyme. The pro-enzyme is matured by proteolysis to yield large (20 kDa) and small (10 kDa) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. To date the regulatory mechanism of caspase-1 activation and the role of caspase-1 in apoptosis are poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive proenzyme form. The recombinant rat caspase-1 was expressed in E. coli as a single polypeptide of 284 amino acids with molecular weight of 34.6. The caspase-1 is purified and activated by proprietary techniques.
Keane K.M.,et al.Cytokine 7:105-110(1995).
Flaws J.A.,et al.Endocrinology 136:5042-5053(1995).
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