|Calculated MW||~50 kDa (monomer). Human full length NAD kinase (aa 1-446) is fused at the N-terminus to a His-tag.|
|Other Names||NADK; Poly (P)/ATP NAD Kinase; EC 188.8.131.52.|
|Results||≥ 1U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1 µmol of NADP per min.|
|Storage||-20°C; 1 mg/ml of ammonium sulphate suspension.|
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Provided below are standard protocols that you may find useful for product applications.
NAD kinase catalyzes the transfer of a phosphate group from ATP to NAD+ to generate NADP+, which in its reduced form acts as an electron donor for biosynthetic reactions. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis.
Lerner F.,et al.Biochem. Biophys. Res. Commun. 288:69-74(2001).
Xu X.,et al.Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Gregory S.G.,et al.Nature 441:315-321(2006).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
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