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Procathepsin L, Human recombinant protein


Product info
Primary Accession P07711
Concentration 1
Calculated MW 39.4 kDa (18-333 aa + N-terminal poly-his tag)
Additional Info
Gene ID 1514
Gene Symbol CTSL1
Other Names CTSL, CTSL1
Gene Source Human
Source E. coli
Assay&Purity SDS-PAGE; ≥90%
Assay2&Purity2 HPLC;
Recombinant Yes
Results > 10000 mU/mg
Target/Specificity Procathepsin L
Format Liquid
Storage -80°C; 1 mg/ml in 25 mM Na2HPO4, 500 mM NaCl, pH 7.0.
Citations (0)

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Cathepsin L is a lysosomal protease that can be secreted from the cell and is implicated to play a role in protein degradation, arthritis, apoptosis, and cancer. BioVision’s Procathepsin L can be auto-catalytically activated to mature Cathepsin L (~24 kDa) by adding an equal volume of 0.2 M NaOAc, 5 mM EDTA pH 4.0 containing fresh 5 mM DTT at RT. The enzyme is fully activated within 30 min of incubation at RT. After activation mature Cathepsin L is highly auto-proteolytic at pH 4.0, and care must be taken to avoid self-proteolysis. If the activated enzyme is not used immediately, methylmethanthiosulfonate (1mM final concentration; MeS-SO2Me; MMTS) could be added and sample should be frozen at -80°C. The activity can be then restored by adding L-cysteine (3 M excess over MMTS) to the enzyme solution.


Gal S.,et al.Biochem. J. 253:303-306(1988).
Joseph L.J.,et al.J. Clin. Invest. 81:1621-1629(1988).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Humphray S.J.,et al.Nature 429:369-374(2004).

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$ 195.00
$ 995.00
$ 4,450.00
Cat# PBV11190r
(40 western blots)
Availability: 5-7days
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