Procathepsin L, Human recombinant protein
CTSL, CTSL1
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P07711 |
---|---|
Concentration | 1 |
Calculated MW | 39.4 kDa (18-333 aa + N-terminal poly-his tag) |
Gene ID | 1514 |
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Gene Symbol | CTSL1 |
Other Names | CTSL, CTSL1 |
Gene Source | Human |
Source | E. coli |
Assay&Purity | SDS-PAGE; ≥90% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Results | > 10000 mU/mg |
Target/Specificity | Procathepsin L |
Format | Liquid |
Storage | -80°C; 1 mg/ml in 25 mM Na2HPO4, 500 mM NaCl, pH 7.0. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Cathepsin L is a lysosomal protease that can be secreted from the cell and is implicated to play a role in protein degradation, arthritis, apoptosis, and cancer. BioVision’s Procathepsin L can be auto-catalytically activated to mature Cathepsin L (~24 kDa) by adding an equal volume of 0.2 M NaOAc, 5 mM EDTA pH 4.0 containing fresh 5 mM DTT at RT. The enzyme is fully activated within 30 min of incubation at RT. After activation mature Cathepsin L is highly auto-proteolytic at pH 4.0, and care must be taken to avoid self-proteolysis. If the activated enzyme is not used immediately, methylmethanthiosulfonate (1mM final concentration; MeS-SO2Me; MMTS) could be added and sample should be frozen at -80°C. The activity can be then restored by adding L-cysteine (3 M excess over MMTS) to the enzyme solution.
References
Gal S.,et al.Biochem. J. 253:303-306(1988).
Joseph L.J.,et al.J. Clin. Invest. 81:1621-1629(1988).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Humphray S.J.,et al.Nature 429:369-374(2004).
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