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Thrombin, Active, Porcine blood recombinant protein

Activated Factor IIa

     
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Product info
Primary Accession F1SIB1
Calculated MW 33.5 kDa
Additional Info
Gene Symbol F2
Other Names Activated Factor IIa
Gene Source Porcine
Source Porcine blood
Assay&Purity SDS-PAGE; ≥98%
Assay2&Purity2 N/A;
Recombinant No
Results 20-1000 IU/mg of lyophilized powder
Target/Specificity Thrombin
Application Notes Reconstitute in sterile water (100 U/ml) with 0.9% NaCl. It forms a clear solution.
Format Lyophilized
Storage -20°C; Sterile filtered and lyophilized without any salts.
Citations (0)

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Background

Thrombin enzyme (Activated Factor IIa) is an important clotting promoter that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. Thrombin triggers factor-XI, factor-V, Factor-XIII and factor-VIII. Thrombin endorses platelet activation, using activation of protease-activated receptors on the platelet. As a result of its high proteolytic specificity, thrombin has become an important biochemical protein. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is widely used in linker regions of recombinant fusion protein constructs. After the purification of the fusion protein, thrombin is used to cleave between the Arginine and Glycine residues of the cleavage site, efficiently removing the purification tag from the protein of interest with a high degree of specificity.

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$ 60.00
$ 225.00
$ 595.00
Cat# PBV11197r
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