|Calculated MW||57.1 kDa|
|Other Names||Protein Disulfide Isomerase, Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase subunit beta, p55|
|Application Notes||Reconstituted PDI should be stored in working aliquots at -20°C. For long-term storage, it is recommended to add carrier protein (0.1% HAS or BSA).|
|Storage||-20°C; Sterile filtered and lyophilized with no additives|
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Provided below are standard protocols that you may find useful for product applications.
Protein disulfide isomerases (PDIs) constitute a family of structurally related enzymes which catalyze disulfide bonds formation, reduction, or isomerization of newly synthesized proteins in the lumen of the endoplasmic reticulum (ER). Human Protein Disulfide Isomerase is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. PDI has been found to have moderate effects (25-fold) on the rate of oxidative folding of proteins in vitro. Recombinant Human PDI produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 508 amino acids and having a molecular mass of 57.1 kDa. The human PDI contains N-terminal 6xHis-tag and is purified by proprietary chromatographic techniques.
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Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
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