|Calculated MW||36.0 kDa|
|Other Names||Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (EC 22.214.171.124) (Peptidyl-cysteine S-nitrosylase GAPDH) (EC 2.6.99.-)|
|Storage||-20°C; 1 mg/ml solution containing 20 mM Tris pH-8, 1 mM EDTA, 1 mM DTT, and 20% glycerol.|
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Provided below are standard protocols that you may find useful for product applications.
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) is a catalytic enzyme normally known to play a role in glycolysis. GAPDH exists as a tetramer composed of 36-kDa subunits and has a range of intracellular functions. GAPDH catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Besides functioning as a glycolytic enzyme in cytoplasm, GAPDH has function in intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. GAPDH catalyzes a vital energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Recombinant human GAPDH produced in E.Coli is a single, non-glycosylated polypeptide chain containing 335 amino acids and having a molecular mass of 36 kDa. The GAPDH is purified by proprietary chromatographic techniques.
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