|Calculated MW||61.9 kDa (NT GST Tag)|
|Other Names||SIR2-like Protein 4; NAD-dependent ADP-ribosyltransferase Sirtuin 4; SIR2L4; Silent Information Regulator 4; Sirtuin 4|
|Storage||-80°C; 50 mM sodium phosphate, pH 7.2, 100 mM sodium chloride, 2.5 mM DTT, 20% glycerol.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
There are seven human sirtuins, also known as class III HDACs, which have been designated SIRT1 to SIRT7. Each is involved in various post-translational modifications by utilizing NAD dependent deacetylase and APD-ribosyltransferase activities. SIRT4 is a mitochondrial ADP-ribosyltransferase responsible for the transfer of ADP-ribose from NAD to specific substrates such as glutamate dehydrogenase (GDH). In caloric-sufficient conditions, SIRT4 ADP-ribosylates GDH, repressing its activity and correspondingly decreasing insulin secretion. There is some uncertainty about the relevance of sirtuin ribosyl transferase activity due to its very slow rate, which is about 500 times lower than the corresponding deacetylation reaction (for those that have been characterized). SIRT4 is found in many tissues, but is specifically enriched in the kidney, heart, brain, and liver.
Frye R.A.,et al.Biochem. Biophys. Res. Commun. 260:273-279(1999).
Scherer S.E.,et al.Nature 440:346-351(2006).
Haigis M.C.,et al.Cell 126:941-954(2006).
Ahuja N.,et al.J. Biol. Chem. 282:33583-33592(2007).
Michishita E.,et al.Mol. Biol. Cell 16:4623-4635(2005).
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.