|Other Names||HeLa Core Histones|
|Source||Highly purified mixture of human core histones (H2A, H2B, H3, and H4) isolated via hydroxyapatite chromatography from HeLa S3 (human cervical adenocarcinoma) nuclear pellet.|
|Storage||-80°C; 50 mM sodium phosphate, pH 6.9, containing 10 mM sodium chloride and 1 mM β-mercaptoethanol.|
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Provided below are standard protocols that you may find useful for product applications.
A nucleosome is the basic repeating unit of chromatin in which 146 base pairs of DNA wrap twice around a histone octamer consisting of two copies of each of the core histones, H2A, H2B, H3, and H4. The combination of two H2A/H2B dimers and one H3/H4 tetramer create the nucleosome core.2 Histone H3 undergoes many modifications which include acetylation, methylation, and phosphorylation that are important for regulation of gene transcription.
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