|Calculated MW||~ 30 kDa|
|Gene Source||E Coli|
|Storage||-20°C; 250 U/ µl in 50% glycerol|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Golden Nuclease is a recombinant form of Serratia macescens extracellular endonuclease produced in Escherichia coli. Golden Nuclease is a homodimer with monomer molecular masses about 30 kDa. Two disulfide bonds found in the nuclease are crucial to its activity and stability. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form (single stranded, double stranded, linear, circular and supercoiled). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5'-phosphorylated oligonucleotides of 3-8 bases in length.
Oshima T.,et al.DNA Res. 3:137-155(1996).
Chung E.,et al.Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
Blattner F.R.,et al.Science 277:1453-1462(1997).
Hayashi K.,et al.Mol. Syst. Biol. 2:E1-E5(2006).
Nonaka G.,et al.Genes Dev. 20:1776-1789(2006).
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