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Benzonase, E. Coli recombinant protein
Endoribonuclease
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P0A898 |
|---|---|
| Calculated MW | ~ 30 kDa |
| Gene ID | 946430 |
|---|---|
| Gene Symbol | YbeY |
| Other Names | Endoribonuclease |
| Gene Source | E Coli |
| Source | E. coli |
| Assay&Purity | SDS-PAGE; ≥98% |
| Assay2&Purity2 | HPLC; |
| Recombinant | Yes |
| Target/Specificity | Benzonase |
| Format | Liquid |
| Storage | -20°C; 250 U/ µl in 50% glycerol |
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Provided below are standard protocols that you may find useful for product applications.
Background
Golden Nuclease is a recombinant form of Serratia macescens extracellular endonuclease produced in Escherichia coli. Golden Nuclease is a homodimer with monomer molecular masses about 30 kDa. Two disulfide bonds found in the nuclease are crucial to its activity and stability. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form (single stranded, double stranded, linear, circular and supercoiled). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5'-phosphorylated oligonucleotides of 3-8 bases in length.
References
Oshima T.,et al.DNA Res. 3:137-155(1996).
Chung E.,et al.Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
Blattner F.R.,et al.Science 277:1453-1462(1997).
Hayashi K.,et al.Mol. Syst. Biol. 2:E1-E5(2006).
Nonaka G.,et al.Genes Dev. 20:1776-1789(2006).
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