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Benzonase, E. Coli recombinant protein

Endoribonuclease

     
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Product info
Primary Accession P0A898
Calculated MW ~ 30 kDa
Additional Info
Gene ID 946430
Gene Symbol YbeY
Other Names Endoribonuclease
Gene Source E Coli
Source E. coli
Assay&Purity SDS-PAGE; ≥98%
Assay2&Purity2 HPLC;
Recombinant Yes
Target/Specificity Benzonase
Format Liquid
Storage -20°C; 250 U/ µl in 50% glycerol
Citations (0)

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Background

Golden Nuclease is a recombinant form of Serratia macescens extracellular endonuclease produced in Escherichia coli. Golden Nuclease is a homodimer with monomer molecular masses about 30 kDa. Two disulfide bonds found in the nuclease are crucial to its activity and stability. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form (single stranded, double stranded, linear, circular and supercoiled). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5'-phosphorylated oligonucleotides of 3-8 bases in length.

References

Oshima T.,et al.DNA Res. 3:137-155(1996).
Chung E.,et al.Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
Blattner F.R.,et al.Science 277:1453-1462(1997).
Hayashi K.,et al.Mol. Syst. Biol. 2:E1-E5(2006).
Nonaka G.,et al.Genes Dev. 20:1776-1789(2006).

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$ 65.00
$ 225.00
Cat# PBV11256r
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