|Calculated MW||~ 30 kDa|
|Gene Source||E Coli|
|Storage||-20°C; 250 U/ µl in 50% glycerol|
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Provided below are standard protocols that you may find useful for product applications.
Golden Nuclease is a recombinant form of Serratia macescens extracellular endonuclease produced in Escherichia coli. Golden Nuclease is a homodimer with monomer molecular masses about 30 kDa. Two disulfide bonds found in the nuclease are crucial to its activity and stability. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form (single stranded, double stranded, linear, circular and supercoiled). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5'-phosphorylated oligonucleotides of 3-8 bases in length.
Oshima T.,et al.DNA Res. 3:137-155(1996).
Chung E.,et al.Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
Blattner F.R.,et al.Science 277:1453-1462(1997).
Hayashi K.,et al.Mol. Syst. Biol. 2:E1-E5(2006).
Nonaka G.,et al.Genes Dev. 20:1776-1789(2006).
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