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Active SIRT2, human recombinant protein
NAD-dependent protein deacetylase sirtuin-2, Regulatory protein SIR2 homolog 2, SIR2-like protein 2,
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q8IXJ6 |
|---|---|
| Calculated MW | Human SIRT2 is a 60 kDa (2 to 389 aa + NT His Tag) protein |
| Gene ID | 22933 |
|---|---|
| Gene Symbol | SIRT2 |
| Other Names | NAD-dependent protein deacetylase sirtuin-2, Regulatory protein SIR2 homolog 2, SIR2-like protein 2, SIR2L, SIR2L2. |
| Gene Source | Human |
| Source | E.coli |
| Assay&Purity | SDS-PAGE; ≥90% |
| Assay2&Purity2 | HPLC; |
| Recombinant | Yes |
| Results | ≥ 4 mU/mg |
| Target/Specificity | SIRT2 |
| Application Notes | Centrifuge the vial prior to opening. Reconstitute in sterile dH2O to a concentration of 0.1 – 1 mg/ml and let the lyophilized pellet dissolve completely. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use. For long term storage, it is recommended to add a carrier protein (0.1% BSA). Avoid multiple freeze/thaw cycles. |
| Format | Sirtuin 2 (SIRT2) with a His-tag is supplied as lyophilized powder. |
| Storage | -20°C; Human Sirtuin 2 is lyophilized from 50 mM Tris, pH 8.0. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Sirtuins function as intracellular regulatory proteins. Human Sirtuin 2 is a member of the class III histone deacetylases (HDACs) and has been implicated in many cellular processes that include histone deacetylation, gene silencing, chromosomal stability, and aging. Human SIRT2 is a cytoplasmic protein responsible for the deacetylation of histone H4 and a-tubulin, a modification important for controlling the cell cycle. Specifically, SIRT2 protein co-localizes with HDAC6 and microtubules and functions as a mitotic checkpoint in preventing chromosomal instability that can lead to hyperploid cells. SIRT2 is found in many tissues, but is specifically enriched in skeletal muscle, the heart, and in oligodendroglia cells in the brain. The enzymatic activity of class III HDACs is nicotinamide adenine dinucleotide (0.1mM NAD+) dependent and insensitive to HDAC inhibitor trichostatin A.
References
Frye R.A.,et al.Biochem. Biophys. Res. Commun. 260:273-279(1999).
Afshar G.,et al.Gene 234:161-168(1999).
De Smet C.,et al.J. Neurochem. 81:575-588(2002).
Rack J.G.,et al.J. Mol. Biol. 426:1677-1691(2014).
Lennerz V.,et al.Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases.
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