Active Transglutaminase 2 (His-tagged), human recombinant protein
TG2, tTG, tissue transglutaminase, transglutaminase C, TGC, protein-glutamine-gamma-glutamyltransfer
|Calculated MW||79.5 kDa (1-687 aa + N-terminal polyhistidine tag).|
|Other Names||TG2, tTG, tissue transglutaminase, transglutaminase C, TGC, protein-glutamine-gamma-glutamyltransferase, TGM2|
|Sequence||1-687 aa of human transglutaminase 2 + N-terminal polyhistidine tag|
|Application Notes||Reconstitute to 0.1-0.5 mU/ µl in 20 mM Tris, 120 mM NaCl, 10 mM DTT, pH 7.5 containing 20% glycerol. Aliquot and store at –80°C. Avoid repeated freezing and thawing cycles.|
|Storage||-80°C; Lyophilized from 5 mg/ml solution in a proprietary buffer.|
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Provided below are standard protocols that you may find useful for product applications.
Tissue transglutaminase, a 78-kDa calcium dependent enzyme (EC 188.8.131.52), is found both in the intracellular and the extracellular spaces of various types of tissues. TG2 crosslinks proteins between the ε-amino group of a lysine residue and the γ-carboxamide group of glutamine residue, creating an inter- or intramolecular bond that is highly resistant to proteolysis (protein degradation). TG2 also possesses deamidation, GTP-binding/hydrolyzing, and isopeptidase activities. Intracellular TG2 is thought to play an important role in apoptosis, while extracellular TG2 has been linked to cell adhesion, ECM stabilization, wound healing, receptor signaling, cellular proliferation, and cellular motility.
Gentile V.,et al.J. Biol. Chem. 266:478-483(1991).
Fraij B.M.,et al.J. Biol. Chem. 267:22616-22623(1992).
Fraij B.M.,et al.Biochim. Biophys. Acta 1306:63-74(1996).
Bayardo M.P.,et al.Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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